ViRNAEx^TM provides fast purification of high-quality RNA from cells collected by standard nasopharyngeal swabbing. Samples can be conveniently delivered in classical viral transport media. A nucleic acid extract that is suitable for this PCR should be used with ViRNAEx^TM. Acceptable specimen types include nucleic acid extracts of nasal swabs, throat swabs, nasopharyngeal swabs, nasopharyngeal aspirate, tracheal aspirate, bronchial washing or bronchoalveolar washing.

RNA purification using the ViRNAEx^TM can be automated on any pipetting automat. For smaller and larger samples, the volume of extracting buffer may be adjusted. The recommended starting volume is 100 µl of sampling solution.

Introduction

ViRNAEx^TM RNA extraction solution enables instant and inexpensive RNA isolation from viruses with a possibility to use RNA subsequently in real time- PCR testing (Volume recommended: 5-10 µl of solution per qPCR reaction). The duration of RNA sample preparation is shortened on approx. 15 minutes including pipetting steps.

Recommended RT-PCR kit

Using CoVirED RT-PCR test for COVID-19 detection in combination with ViRNAEx^TM is strongly recommended. CoVirED PCR protocol includes internal PCR reaction control as well as control of human cells presence using human endogenous control gene probe.  CoVirED (Corona Virus Extraction and Detection kit) is delivered for a favorable price if combined with ViRNAEx^TM (one package). More info here.

Package:

ViRNAEx^TM – 10 mL (for 100 rxns)
ViRNAEx^TM – 50 mL (for 500 rxns)

CE IVD

Comparison of the virus RNA detection from different matrices
Fresh (1) vs. Frozen (2) virus transfer media using ViRNAEx^TM RNA-extraction protocol     

Compared viral RNA isolation protocols (magnetic beads-based method, column-based method) to ViRNAEx^TM extraction protocol in fresh and frozen virus transfer media of patients tested previously as COV2- positive. Obtained RNA has been used for GeneFirst™ qPCR testing of the genes:  SARS-CoV-2- Gene N (A), SARS –COV2- ORF1ab (B), and epithelial cell control gene (C).

Analyzing the data from qPCR:

Figure 1: ViRNAEx^TM has comparable RNA-virus detection capacity if fresh virus transfer media is used for RNA isolation. The differences in Ct  values  were described on 24 samples and represent difference of

A. 1,7(Ct- cycle) for SARS –COV2-N gene)

B. 1,6 (Ct-cycle) for SARS-COV2-OFR1ab gene  and 

C. 3,3 (Ct- cycle) for epithelial control gene. Please see the Figure 1 A, B, C. The green amplification curve represents ViRNAEx^TM sample, the red one represents RNA separated by magnetic beads.

Figure 2: Similarly, on the Figure 2  the ViRNAEx^TM (Green , Red curve) is compared to the column-based RNA isolation (blue curve). In this case frozen virus transfer media of previously positive patients were used for comparisons (12 samples in total). We may see the significant delays in SARS-COV2- N gene (8 Ct cycles difference)  and SARS- COV2 – ORF1ab gene (10 Ct cycles  difference). The  storage under freezing conditions did not influence the epithelial control gene detection.  Green and red curves represent different starting concentrations of viral load.

Safety Data Sheet (SDS) Information Sheet:

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